Method of preparing microorganism producing butanol with elevated butanol tolerance

摘要

The present invention relates to a method of producing butanol resistance and enhanced butanol production strain and , specifically, Claus Stadium bay tree that linkage ( Clostridium beijerinckii ) NCIMB 8052 was mutated to wild-type strain , and then had to have a cell membrane synthesis inhibitor -resistant chemical , selected by screening a strain which can produce a high concentration of butanol , butanol resistance and high butanol relates to a production strain . Butanol resistance and high butanol production strain prepared by the method of the present invention can be used very usefully in biobutanol commercialized technology .