METHOD FOR PREPARING STRAIN WHICH HAS BUTANOL TOLERANCE AND IS ABLE TO PRODUCE BUTANOL WITH HIGH YIELD THROUGH MEASUREMENT OF BUTANOL CONTENT IN SAMPLE

摘要

PURPOSE: A method for preparing strain which has enhanced butanol tolerance and produced butanol with high yield is provided to usefully use in biobutanol commercialization.;CONSTITUTION: A method for measuring butanol content in a sample comprises: a step of reacting reagent containing alcodhol dehydrogenase, reaction buffer, and co-enzyme NAD+; a step of measuring absorbance of reagent at 340nm; a step of performing the reaction using a sample which possibly contains butanol; a step of measuring absorbance of the sample; and a step of comparing standard curve and calculating butanol content.;COPYRIGHT KIPO 2010