Galactosidase with alpha-galactosyl transferase activity

摘要

1. The DNA sequence that! a) encodes a protein with the amino acid sequence given in SEQ ID NO: 2, or! b) hybridizes under stringent hybridization conditions with the sequence a) or! c) is a degenerate derivative of the sequence a) or b). ! 2. The DNA sequence according to claim 1, which is given in SEQ ID NO: 1 or in its fragment. ! 3. The DNA sequence according to claim 1 or claim 2, which contains several substitutions, insertions or deletions that result in less than 60%, preferably less than 45%, more preferably less than 25% of the changes in the amino acid sequence, corresponding to SEQ ID NO: 2 or a fragment thereof. ! 4. The DNA sequence according to claim 1 or claim 2, wherein said sequence includes nucleotide substitutions, which result in conservative amino acid substitutions. ! 5. The enzyme encoded by the DNA sequence according to any one of claims 1 to 4. ! 6. The enzyme containing the amino acid sequence corresponding to SEQ ID NO: 2 or its fragment. ! 7. β-galactosidase with the sequence defined in SEQ ID NO: 2.! 8. Recombinant vector containing the DNA sequence according to any one of claims 1 to 4. ! 9. The vector of claim 8, wherein said vector is an expression vector. ! 10. A host cell containing the DNA sequence according to any one of claims 1 to 4. ! 11. A host cell containing the vector of claim 8 or claim 9. ! 12. The host cell of claim 10 or claim 11, in which the specified cell is a bacterial cell, a yeast cell or a fungal cell. ! 13. The host cell of claim 12, wherein said cell is selected from the group consisting of Bifidobacterium, Lactococcus, Lactobacillus, Escherichia, Bacillus and Aspergillus. ! 14. A host cell according to claim 13, in which the cell is selected from the group consisting of