METHOD OF IHTIOVIRUSES ISOLATION FROM CLINIC MATERIAL OF FISH FOR DIAGNOSTIC AND MONITORING RESEARCH

摘要

FIELD: medicine.;SUBSTANCE: particles of covering tissues, which are applied for virus isolation, after preliminary washing in medium with increased content of antibiotics, are placed in bottles with nutrient medium - medium EMEM or medium 199 with 2% of cow foetus blood serum and 200 U/ml of penicillin, 200 mcg/ml of streptomycin and 25 mcg/ml of nystatin. Incubation is carried out during 3-5 days at optimal temperature for virus reproduction and constant swing on shaker (50 swings per min.). After that virus separation from tissue is carried out.;EFFECT: application of method allows to increase virus separation efficiency, allows to separate virus in research of clinical material of fishes which had disease at late stage of epizooty, material of fish-virus carriers in inter-epizooty period and in monitoring of sturgeon and carp fish population for presence of herpes-viral disease stimulant and spring viremia, when probability of virus separation by known method is low.;2 ex, 4 tbl, 1 cl