METHOD FOR LABELING NUCLEIC ACID IN SEQUENCE-SPECIFIC MANNER, AND NEW METHOD FOR DETECTING NUCLEIC ACID USING THE SAME

摘要

PPROBLEM TO BE SOLVED: To provide a technique by which a nucleic acid can simply be detected. PSOLUTION: The invention relates to a method for producing a nucleic acid having an introduced label, comprising the steps of, (A) providing a nucleic acid to be labeled and a template nucleic acid, the template nucleic acid being complementary to at least a portion of the nucleic acid to be labeled, and has a nucleotide which is longer by the length of at least one nucleotide than the labeling target nucleic acid on at least one end of the labeling target nucleic acid, (B) hybridizing the nucleic acid to be labeled with the template nucleic acid to produce a nucleic acid to be labeled-template nucleic acid complex, (C) subjecting the nucleic acid to be labeled-template nucleic acid complex to an extension reaction, in which a nucleic acid synthesizing enzyme and labeled nucleotides are used, so that at least one of the labeled nucleotides is incorporated into the nucleic acid to be labeled based on the template nucleic acid, and (D) recovering the elongated nucleic acid to be labeled from the resultant mixture, after the extension reaction. PCOPYRIGHT: (C)2011,JPO&INPIT