Generation of recombinant genes in prokaryotic cells by using a two extrachromosomal elements

摘要

Overall, the use of the extrachromosomal element two different, in prokaryotic cells, and the bacteria in particular, a method for generating and detecting recombinant DNA sequences, and the present invention is to carry out the methods of the present invention extrachromosomal element may be used, on the plasmid, for example. DNA sequences that this method is concerned, including the non-coding sequences and protein coding sequence.