Process for the isolation of polyhydroxyalkanoates (PHAs) from a cell mass by solvent extraction involving a step of treating the PHA solution with water and/or steam for evaporation of the PHA solvent and precipitation of the PHA

摘要

A process for extracting and recovering polyhydroxyalkanoates (PHAs) fromcellular biomass comprising the steps of: extracting the polyhydroxyalkanoatewith at least one non-halogenated solvent which is non-aggressive to the environment;heating the cellular biomass, in order to form a suspension comprising PHA solventwith the dissolved PHA and insoluble residues; recovering the solvent enrichedwith PHA; injecting the PHA-enriched solvent in a vapor flow in order to rapidlypromote the complete precipitation of PHA in water, while processing the solventevaporation; depleting the residual solvent; separating the purified PHA particlesfrom the suspension and drying them.