INTEGRATED SAMPLE PREPARATION AND AMPLIFICATION FOR NUCLEIC ACID DETECTION FROM BIOLOGICAL SAMPLES

摘要

Beads are used to perform both a sample preparation and amplification process within a single processing chamber. A fluid sample including a plurality of cells is introduced into a processing chamber already including a plurality of beads. The plurality of cells are lysed within the processing chamber, and nucleic acid released from the lysed cells binds to the beads. The beads are retained in the processing chamber while the lysing reagents are removed. Amplification reagents are added to the processing chamber and an amplification process is performed on the contents of the processing chamber. Amplification of the captured nucleic acid is performed while the nucleic acid is still bound to the beads. During the amplification process, the nucleic acid bound to the beads is amplified. Real-time or end-point detection can be performed on the content of the processing chamber to detect the presence of amplified product.