METHOD FOR PRODUCTION OF 3-HYDROXYPROPIONIC ACID FROM GLYCEROL BY CULTIVATING RECOMBINANT E. COLI

摘要

PURPOSE: A method for efficiently preparing 3-hydroxypropinoic acid from glycerol using recombinant E.coli is provided. CONSTITUTION: A method for preparing 3-hydroxyproionic acid from glycerol comprises a step of culturing recombinant E.coli in a medium containing glycerol and rich glucose. A method for preparing the recombinant E.coli comprises: a step of transforming E.Coli to express Lactobacillus brevis-derived glycerol dehydratase and Lactobacillus brevis-derived glycerol dehydratase reactivase; and a step of expressing an enzyme which converts 3-hydroxypropin aldehyde to 3-hydroxypropionic acid.