A SET OF DIFFERENTIATING AND SPECIFIC OLIGONUCLEOTIDES FOR IDENTIFICATION OF DNA OF ACUTE INTESTINAL INFECTIONS, METHOD FOR IDENTIFICATION OF OKA, MICROCHIP AND DIAGNOSIS OF THE SYNTHESIS

摘要

1. A set of specific oligonucleotides for rapid analysis of species identification of pathogens of acute intestinal infections, the sequence of which is defined in the List of sequences (SEQ ID NO: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17 ) ! 2. A set of differentiating oligonucleotides for rapid analysis of species identification of pathogens of acute intestinal infections, the sequence of which is defined in the List of sequences (SEQ ID NO: 3, 6, 9, 12, 15, 18, 19). ! 3. A set of recombinant plasmids for rapid analysis of species identification of pathogens of acute intestinal infections, a list of which is given in table 1. ! 4. A biochip for express analysis of species identification of pathogens of acute intestinal infections, which is a substrate with a set of oligonucleotides immobilized on it, described in paragraph 2. ! 5. A method for the species identification of pathogens of acute intestinal infections in a clinical sample obtained from a patient with acute intestinal infections, comprising the following stages:! (a) the preparation of the biological microchip described in claim 4, which consists in modifying the surface of the substrate and in the subsequent immobilization of the differentiating oligonucleotides described in claim 2; ! (b) the implementation of DNA extraction from a clinical sample and the use of a recombinant plasmid, characterized in paragraph 3 to control the process of DNA extraction; ! (c) carrying out multiplex symmetric polymerase chain reactions (PCR) of samples of isolated DNA using a kit containing biotin-labeled primers described in paragraph 1, as well as additional conducting symmetric PCR with a set of recombinant plasmids characterized