DETECTION METHOD OF DNA OF TUBERCULOSIS CAUSATIVE AGENT WITH SIMULTANEOUS IDENTIFICATION OF ITS GENOTYPE AND DETERMINATION OF GENETIC DETERMINANTS OF MULTIPLE AND WIDE DRUG RESISTANCE, OLIGONUCLEOTIDE MICROCHIP, SET OF PRIMERS AND SET OF OLIGONUCLEOTIDE PROBES, WHICH ARE USED IN METHOD

摘要

FIELD: biotechnologies.;SUBSTANCE: invention relates to a detection method of DNA of a tuberculosis causative agent -mycobacteria of tuberculosis complex with simultaneous identification of a genotype of the causative agent and determination of genetic determinants of resistance to a wide spectrum of antituberculous remedies, including rifampicin, isoniazid, fluoroquinolones, kanamycin, capreomicin, amikacin, ethambutol, in a clinical specimen on a differentiating oligonucleotide microchip. The method is based on a multiplex PCR with simultaneous marking of PCR products with further hybridisation of the obtained products on the oligonucleotide microchip containing a set of specific discriminating oligonucleotides. The invention also deals with an oligonucleotide microchip, a set of primers and a set of oligonucleotide probes, which are used for the method's implementation.;EFFECT: invention allows performing an analysis of DNA separated directly from a clinic specimen, detecting genotypes of a tuberculosis causative agent, which are associated with increased virulence and transmissibility, providing personified selection of effective antituberculous remedies for each certain patient and performing epidemiological genetic typing.;9 cl, 7 dwg, 3 tbl, 8 ex