METHOD OF PRODUCING AND RECORDING MARKED BACTERIOPHAGES IN CHOLERA GERM MODEL

摘要

FIELD: medicine.;SUBSTANCE: donor strain producing an isotope-marked bacteriophage which is pre-recovered from lysogenic strain V.cholerae eltor in the 1 concentration n·10⁸ PFU/ml is produced and added with 5 mcc/ml of isotope ³H-thymidine, and kept for one day in a thermostat at 37°C to produce marked cholera phage. The latter is introduced in the amount 1 ml in a test tube with an indicator recipient strain in the ratio 1:1 for lysogenisation, incubated at 37°C within one day; 0.1 ml of the derived mixture is seeded on an agar plate, grown at 37°C within 20-24 hours, and the donor strain is recovered. The residual portion of the mixture is used to record radioactivity of the bacteriophage and recipient strain. The positive result is shown by radioactivity of the marked bacteriophages within the range 475-1269 p/min, and of the recipient strains within the range 78-103 p/min.;EFFECT: invention allows higher efficiency of detection and recording of the cholera phage in a ³H-thymidine marked cholera germ cell.;3 tbl, 4 ex