Preparing glucose solution from starch comprises e.g. transferring starch milk into liquefaction container containing amylase, maintaining at specified temperatures, and transferring into saccharification container comprising glucoamylase

摘要

Preparing a solution of glucose from starch, comprises: mixing starch with water to form starch milk and transferring into a liquefaction container comprising alpha -amylase; maintaining in the liquefaction container in a first time period at a first temperature and a second time period at a second higher temperature; transferring liquefied starch into a saccharification container comprising glucoamylase, which separates the formed glucose solution into a sterile, glucose-containing and enzyme-free permeate and non-reacted starch and enzyme-containing retentate; and recycling retentate. Preparing a solution of glucose from starch, comprises: mixing the starch with water to form a starch milk and transferring into a liquefaction container comprising a first enzyme or enzyme system of alpha -amylase with natural adjustment of pH without addition of acid; maintaining in the liquefaction container initially in a first time period at a first temperature and then over a second time period at a second higher temperature; cooling from the second temperature to a third, lower temperature; transferring the liquefied starch into a saccharification container comprising a second enzyme or an enzyme mixture of glucoamylase, which separates the formed glucose solution, in an ultrafiltration, into a first stream of a sterile, glucose-containing and practically enzyme-free permeate and a second stream of non-reacted starch and enzyme-containing retentate; and recycling the retentate into the saccharification container in which the concentration of enzyme is maintained at high, stationary level.