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Method for enhancing the secretion efficiency of recombinant foreign proteins in yeast expression systems
Method for enhancing the secretion efficiency of recombinant foreign proteins in yeast expression systems
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机译:提高酵母表达系统中重组外源蛋白分泌效率的方法
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摘要
To provide a method for improving secretion efficiency of recombinant foreign proteins in yeast expression systems. The method, it can be transformed with a recombinant foreign gene construct comprising a gene encoding a foreign protein galactose-inducible promoter, secretion signal sequence, the yeast host, to construct a transformed yeast strain; transformed yeast and It comprises the step of culturing under conditions where the activity of the galactose-inducible promoter is controlled, and strain. Improvement of the secretion efficiency of a foreign protein, excess recombinant foreign protein through appropriate control of the levels of galactose acts as inducer of the galactose-inducible promoter in cells suffer the yeast expression system based on galactose-inducible promoter of the conventional can be achieved by reducing the insoluble precipitate-induced expression. Improved secretion efficiency of recombinant foreign protein, the present invention contributes to the reduction of production cost and improve the production of recombinant foreign proteins in the yeast expression system.
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