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Modified family 6 glycosidases with altered substrate specificity
Modified family 6 glycosidases with altered substrate specificity
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机译:修饰的家族6糖苷酶具有改变的底物特异性
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摘要
A modified Family 6 glycosidase enzyme comprising amino acid substitutions at one or more positions selected from the group 182, 367, 399, 400 and 427 is provided (the position determined form alignment of a parental Family 6 glycosidase with SEQ ID NO: 1). Genetic constructs and genetically modified microbes comprising nucleic sequences encoding the modified Family 6 glycosidase are also provided. Family 6 glycosidase of the invention display decreased hydrolysis activity of beta 1-4 linked polysaccharides and increased hydrolysis activity of beta 1-3, 1-4 linked polysaccharides compared with a parental Family 6 glycosidase. Such glycosidases find use in a variety of applications in industry, e.g., in hydrolysis of beta 1-3, 1-4 linked polysaccharides during the processing of cereal grains or the production of alcohol, animal feed or food products.
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机译:提供了修饰的家族6糖苷酶,其在选自182、367、399、400和427的组的一个或多个位置上包含氨基酸取代(该位置由亲本家族6糖苷酶与SEQ ID NO:1的比对确定)。还提供了包含编码修饰的家族6糖苷酶的核酸序列的遗传构建体和经遗传修饰的微生物。与亲本的家族6糖苷酶相比,本发明的家族6糖苷酶显示出降低的β1-4连接的多糖的水解活性和增加的β1-3、1-4连接的多糖的水解活性。此类糖苷酶可用于多种工业用途,例如在谷物加工或生产酒精,动物饲料或食品时水解β1-3、1-4连接的多糖。
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