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METHOD OF ANALYZING PROTEIN USING DATA-INDEPENDENT ANALYSIS COMBINED WITH DATA-DEPENDENT ANALYSIS
METHOD OF ANALYZING PROTEIN USING DATA-INDEPENDENT ANALYSIS COMBINED WITH DATA-DEPENDENT ANALYSIS
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机译:数据独立分析与数据独立分析相结合的蛋白质分析方法
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摘要
The present invention relates to a method of analyzing a protein or proteins comprising the steps of: (A) pre-treating a mixture containing at least one protein to obtain peptides; (B) obtaining information about retention times and mass values of the obtained peptides by performing data-independent analysis; (C) searching a first database on the basis of the information obtained in step (B) to quantify and qualify a target protein or proteins; (D) extracting information about the quantified and qualified target protein or proteins; (E) obtaining information about retention times and mass values by performing data-dependent analysis from the extracted information of step (D); (F) searching a second database on the basis of the information obtained in step (E) to further quantify and qualify the target protein or proteins; and (G) comparing the search results of step (C) and (F) to verify the quantification and qualification.
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