首页>
外国专利>
Method for detection and multiple, simultaneous quantification of pathogens by means of real-time polymerase chain reaction
Method for detection and multiple, simultaneous quantification of pathogens by means of real-time polymerase chain reaction
展开▼
机译:实时聚合酶链反应检测和同时定量病原体的方法
展开▼
页面导航
摘要
著录项
相似文献
摘要
A method for multiple and simultaneously detecting and quantifying of Listeria spp, Staphylococcus aureus, Campylobacter jejuni, and Escherichia coli 0157:H7, by extracting DNA from a sample; preparing a reaction mixture for enzymatic amplification of the extracted DNA and sets of pairs of oligonucleotide primers identified as SEQ ID No. 1 and SEQ ID No. 2, SEQ ID No. 4 and SEQ ID No. 5, SEQ ID No. 7 and SEQ ID No. 8, and SEQ ID No. 10 and SEQ ID No. 11, and probes with oligonucleotide sequences identified as SEQ ID No. 3, SEQ ID No. 6, SEQ ID No. 9 and SEQ ID No. 12; providing a thermostable DNA polymerase and magnesium salt to the reaction mixture; amplifying the reaction mixture by a PCR reaction; and determining the presence or absence, and quantification of the pathogens by using a fluorescent signal or fluorescence emission specific for each pathogen.
展开▼
机译:通过提取DNA来同时检测和定量李斯特菌 I> spp,金黄色葡萄球菌,空肠弯曲杆菌 I>和大肠杆菌 I> 0157:H7的方法从样本中制备用于酶促扩增提取的DNA的反应混合物以及标识为SEQ ID No.1和SEQ ID No.2,SEQ ID No.4和SEQ ID No.5,SEQ ID No.7和SEQ ID No.8,SEQ ID No.10和SEQ ID No.11,以及具有标识为SEQ ID No.3,SEQ ID No.6,SEQ ID No.9和SEQ ID No.12的寡核苷酸序列的探针;向反应混合物提供热稳定的DNA聚合酶和镁盐;通过PCR反应扩增反应混合物;通过使用对每种病原体特异的荧光信号或荧光发射,确定病原体的存在与否,并对病原体进行定量。
展开▼