Nucleic acid primers, probe and method for detection of neisseria gonorrhoeae.

摘要

The present invention relates to nucleic acid primers and probe for detection of Neisseria gonorrhoeae. The use of the probe sequence for detection of N. gonorrhoeae in clinical samples (endocervical swabs in females and urethral discharge in males) has been described along with the different biomaterials to which it can be immobilized for detection purpose by the biosensor technology. In addition to its use as a detection probe, the sequence can be used as primer for in vitro amplification of N. gonorrhoeae in clinical samples. The discriminatory capacity of the unique sequence has been established by utilizing the panel of non-N. gonorrhoeae Neisseria species (NgNS) as well as other gram-negative bacteria.