PLASMIDIC GENE CONSTRUCTION (PMTTSLUCFF) INCLUDING THE PROMOTER OF THE MTT5 GENE OF THE TETRAHYMENUS THERMOPHILA CYLINDER, FOR THE ELABORATION OF A USEFUL CELLULAR BIOSENSOR IN THE DETECTION OF HEAVY METALS.

摘要

Plasmid gene construct pMTT5LucFF, constructed from plasmid pBt {dl} -7Luc, after inserting the PMTT5 :: lucFF construct. This construct is formed by the promoter region of the MTT5 gene (coding for a Cd-metallothionein) of the T. thermophila ciliated and the LucFF reporter (luciferase coding) gene. # This plasmid is the base molecular tool for the construction of a biosensor. cell in the ciliated Tetrahymena thermophila model, which is useful for the detection of heavy metals in presumably contaminated aquatic and / or terrestrial samples.