pincipales hybrid proteins of Parietaria Judaica allergen and uses thereof

摘要

hybrid protein consisting of allergens Par j 1 and Par j 2 mutated, optionally separated by unligador, wherein: a) the sequence of Par j 1 or is SEQ ID NO: 1 or a sequence of a protein produced identical naturally demanera to SEQ ID NO: 1 in at least 95%, yen said sequence of Par j 1, residues Lys at positions 23, 27, 41 and 66 of SEQ ID NO: 1, or inthe corresponding positions said protein occurs naturally in which estánpresentes Lys residues corresponding alignments of respective sequences are replaced poraminoácidos neutral polar or acidic b) the sequence of Par j 2 or is SEQ ID NO: either 2 or sequence of a protein identical naturally demanera occurs SEQ ID NO: 2 by at least 95%, yen said sequence of Par j 2, residues Lys 19, 23, 27, 35, 41, 46 positions, 73 and 78 of SEQID NO: 2, or the corresponding positions of said protein occurs so natura l in Lasque present Lys residues corresponding are alignments of respective sequences, sesustituyen by neutral polar or acidic amino acids; c) sequences Par j 1 and Par j 2 as defined above, can be located either inthe N- or C terminals of the hybrid protein, provided they are oriented head to tail, d) if present, said linker consists of from 1 to 8 amino acids.