DELIVERY OF NUCLEIC ACIDS INTO EUKARYOTIC GENOMES USING IN VITRO ASSEMBLED MU TRANSPOSITION COMPLEXES

摘要

The present invention relates to genetic engineering and especially to the useof DNA transposition complex of bacteriophage Mu. In particular, the inventionprovides a gene transfer system for eukaryotic cells, wherein in vitroassembled Mu transposition complexes are introduced into a target cell andsubsequently transposition into a cellular nucleic acid occurs. The inventionfurther provides a kit for producing insertional mutations into the genomes ofeukaryotic cells. The kit can be used, e.g., to generate insertional mutantlibraries.