Dried Composition for hot-start PCR with Long-Term Stability

摘要

The present invention was added to pyrophosphate and fatigue phosphatase in an aqueous solution containing relates to a dry composition for hot start PCR, more particularly a buffer solution for reaction in the one reaction tube, MgCl _2, four kinds of dNTP, DNA polymerase preparing a reaction mixture, and relates to the reaction mixture to drying by the characterized in that the manufacturing stability and long term storage property is improved hot start PCR-dried composition and production method the nucleic acid amplification method using the same. Hot Start-dried composition for PCR of the present invention are pyrophosphate and by the addition of fatigue phosphatase at the same time and dried has improved ease of use, reliability and long-term storage as compared to conventional hot-start PCR composition, hot start PCR, multiplex PCR, or can be effectively used, etc. Real-time quantitative PCR method. ; Hot Start PCR, pyrophosphate, fatigue phosphatase, stabilized PCR, quantitative PCR