MULTIPLEX PCR PRIMER COMPOSITE FOR THE DETECTION OF BACILLUS CEREUS EMETIC TOXIN AND KIT

摘要

PURPOSE: A multiplex PCR primer composition and a kit for detecting toxin gene of Bacillus cereus are provided to quickly and accurately detect emetic toxin. CONSTITUTION: A multiplex PCR primer composition for detecting toxin gene of Bacillus cereus contains a primer of sequence numbers 1-6. The toxin gene is CER, ces, or groEL. A kit for detecting the toxin gene contains the primer and a reagent for amplification. The reagent contains DNA polymerase, dNTPs, and buffer.