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QUANTITATIVE ANALYSIS OF HEMAGGLUTININ OF INFLUENZA VACCINE
QUANTITATIVE ANALYSIS OF HEMAGGLUTININ OF INFLUENZA VACCINE
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机译:流感疫苗血凝素的定量分析
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摘要
PURPOSE: A quantitative analysis of hemagglutinin of influenza vaccine is provided to quantifying the hemagglutinin of influenza vaccine without standard antigen and standard antibody.;CONSTITUTION: A quantitative analysis of hemagglutinin of influenza vaccine comprises the following steps: separating a standard antigen the concentration in which hemagglutinin concentration is known by using the size exclusion chromatography; separating influenza vaccines in which the hemagglutinin concentration is not know by using the size exclusion chromatography under the same condition as the first step; calculating area of peaks which show the antigen-antibody reactions by using the result of the size exclusion chromatography of the standard antigen in the first step; calculating the area of peaks corresponding to the peaks in the third step by using the size exclusion chromatography result of the influenza vaccine; multiplying by the hemagglutinin return concentration of the standard antigen in the first step after dividing the outcome of the fourth step into the outcome of the third step; and calculating concentration correcting value which is the difference of converted value of each peak area, in which the antigen-antibody reaction is displayed based on 1 micro gram of hemagglutinin performing the size exclusion chromatography is using the standard antigen provided in the first step and performing the size exclusion chromatography by using the influenza vaccine which can checks the concentration of the hemagglutinin; and dividing the result of the fifth step by the concentration correction value of the hemagglutinin of the influenza vaccine in the sixth step.;COPYRIGHT KIPO 2013
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