A novel Hansenula polymorpha gene coding for dolichyl-phosphate-mannose dependent ?-1,3-mannosyltransferase and process for the production of recombinant glycoproteins with Hansenula polymorpha mutant strain deficient in the same gene

摘要

The present invention relates to a method of production using a century Cronulla poly Maurepas system human-type glycoprotein that is reduced by operating the glycosylation enzymes involved in sugar chain formed by genetic engineering. More specifically to a century Cronulla Maurepas poly alpha-1,3-dependent mannose phosphate turn seven million nosil transferase (dolichyl-phosphate-mannose dependent α-1,3-mannosyltransferase) identified the gene defect and the identification of the genes producing a century Cronulla poly Maurepas mutant producing a glycoprotein with reduced glycosylation and is related to a method for producing a glycoprotein having a human-type sugar chain through a variety of operations of the mutant strain. ; Yeast, poly-century Cronulla Maurepas, protein, sugar chain redesign per recombinant