LIPID MODIFIED TWO-RADIUS RNA HAVING A STRONG EFFECT OF RNA INTERFERENCE

摘要

1. A lipid-modified double-stranded RNA containing a sense strand having a nucleotide sequence complementary to a target sequence in a target gene, and an antisense strand having a nucleotide sequence complementary to a sense strand, wherein the double-stranded RNA is capable of inhibiting target gene expression, and the sense strand has a lipid attached directly or via a linker, to at least one of the first six nucleotides at the 5'-end. ! 2. The lipid-modified double-stranded RNA according to claim 1, blunt-ended on the 5'-end side of the sense strand, and blunt-ended or having a protruding end on the 3'-end side of the sense strand. ! 3. The lipid-modified double-stranded RNA according to claim 1, having protruding ends simultaneously on the 5'- and 3'-terminal sides of the sense strand. ! 4. The lipid-modified double-stranded RNA according to any one of claims 1 to 3, in which the sense strand consists of 21-27 nucleotides. ! 5. The lipid-modified double-stranded RNA according to claim 2, obtuse at the same time on the 5'- and 3'-terminal sides of the sense strand, and in which each of the sense and antisense strands consists of 27 nucleotides. ! 6. The lipid-modified double-stranded RNA according to claim 2, obtuse at the same time on the 5'- and 3'-terminal sides of the sense strand, and in which each of the sense and antisense strands consists of 23 nucleotides. ! 7. The lipid-modified double-stranded RNA according to claim 2, blunt at the 5'-end side of the sense strand, the sense strand consisting of 25 nucleotides, and the antisense strand consisting of 23 nucleotides. ! 8. The lipid-modified double-stranded RNA according to claim 3, in which each of the semantic and antism