METHOD FOR DETERMINING INDICATOR TOTAL antioxidant activity of biological objects by cathodic voltammetry

摘要

FIELD: medicine.;SUBSTANCE: invention describes voltammetric method of determining total activity of antioxidants, which includes process of substance reduction on electrode in electrolyte solution, where as model reaction used is process of electric reduction of oxygen, which is carried out on glassy-carbon electrode at the background phosphate buffer pH 6.86 in water medium, 0.1M NaCIO₄ in aprotonic medium or 0.9% NaCl in blood serum, registration of cathode waves of one-electron reduction of oxygen is carried out in mode of differentiation at rate of potential sweep 50 mV/s, total antioxidant activity is determined by relative change of current of oxygen electric reduction within potential range from 0 to 1.0 V from the time of interaction of total content of antioxidants in sample with oxygen and its active radicals (t) with application of kinetic criterion.;EFFECT: method makes it possible to increase sensitivity, accuracy, rapidity of determination, requires small sample preparation with use of dissolved in background solution oxygen, method makes it possible to estimate optimal doses of one or another preparation which possess the greatest antioxidant activity, determine stability of tested samples in time.;1 ex, 3 tbl, 2 dwg