METHOD FOR DETERMINING TOTAL CONTENT OF THIOL ANTIOXIDANTS IN PLANT OBJECTS BY CATHODIC VOLTAMMETRY

摘要

FIELD: chemistry.;SUBSTANCE: method involves plotting current versus voltage curves of thiol compounds using a glassy-carbon electrode at potential -0.4 V relative a saturated silver chloride electrode with direct current form of potential at a rate of 0.05 V/s, and pH of the borate buffer solution equal to 9.18. The determined content of thiol compounds ranges from 1.0·10^-9 mol/l to 2.0·10^-8 mol/l. The invention can be used as a test for the effect of heavy metals, which enables to assess the state of the environment based on content of SH-groups in proteins.;EFFECT: high sensitivity and rapidness of determining total content of thiol antioxidants in plant material.;1 tbl, 1 ex, 2 dwg