The nutrient medium for accelerated drug susceptibility M. TUBERCULOSIS CORE TB drugs

摘要

The nutrient medium for the rapid determination of drug susceptibility of M. tuberculosis to the main antituberculosis preparations containing nutritional dry basis commercial Middlebrook 7H9 broth medium, characterized in that it comprises additionally 0.125% microbiological agar with the following component composition: 4.7 g of dry Middlebrook 7H9 broth, which is composed, g / l: Ammonium sulfate 0.5! L-glutamic acid 0.5! Sodium citrate 0.1! Pyridoxine 0,001! Biotin 0.0005! Sodium phosphate dibasic 2.5! Potassium phosphate monobasic 1.0! Iron limonnoammoniynoe 0.04! Magnesium sulfate 0.05! Calcium chloride is 0.0005! Zinc sulfate 0.001! Copper sulfate 0.001! Glycerol 2ml! Pancreatic casein hydrolyzate 1.25! Agar Microbiological 1.25! Distilled water 900 ml! 100 ml of growth supplements OADC, which comprises: Sodium chloride 8.5 g / l! Bovine albumin (fraction 5) 50.0 g / l! Glucose 20.0 g / l! Catalase 0.03 g / l! Oleic acid 0.6 ml / l