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Method for testing drug susceptibility of tuberculosis bacteria, using indicator, and solid medium

机译:用指示剂和固体培养基测试结核菌药物敏感性的方法

摘要

A testing method for drug sensitivity of Mycobacterium tuberculosis , application of the indicator used in the method, solid medium and constant temperature heater and culture box used in the method are provided. The testing method for drug sensitivity of Mycobacterium tuberculosis comprises following steps: pretreating the provided specimen, and then adding liquid medium to conduct enrichment culturing, heating the solid medium to melt to prepare the solid medium in liquid state, mixing the specimen after enrichment culturing with the solid medium in liquid state, cooling to solid state, and preparing the solid drug sensitivity test specimen with drug sensitivity paper, culturing it for 5-15 days, observing the drug inhibiting ring by using an indicator to verify the drug sensitivity. The method costs less time for test and enables to perform drug sensitivity test of Mycobacterium tuberculosis in a safe and cost-effective manner.
机译:提供了结核分枝杆菌药物敏感性的测试方法,该方法所用指示剂的应用,该方法所用的固体培养基和恒温加热器以及培养箱。结核分枝杆菌药物敏感性的测试方法包括以下步骤:预处理所提供的标本,然后加入液体培养基进行浓缩培养,加热固体培养基使其熔融以制备液态固体培养基,将浓缩培养后的样本与将固体介质呈液态,冷却至固态,用药物敏感性纸制备固体药物敏感性测试样品,培养5-15天,用指示剂观察药物抑制环,以验证药物敏感性。该方法花费了更少的测试时间,并且能够以安全且成本有效的方式执行结核分枝杆菌的药物敏感性测试。

著录项

  • 公开/公告号JP2013526874A

    专利类型

  • 公开/公告日2013-06-27

    原文格式PDF

  • 申请/专利号JP20130512728

  • 发明设计人 彭▲鈞▼;

    申请日2011-05-30

  • 分类号C12Q1/04;C12N1;C12M1/34;C12M1;

  • 国家 JP

  • 入库时间 2022-08-21 17:01:01

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