METHOD FOR PRODUCING ERYTHROCYTIC MEMBRANES WITH RESERVED LONG STORAGE MORPHOFUNCTIONAL STATUS

摘要

FIELD: medicine.;SUBSTANCE: method for producing erythrocytic membranes, consisting that haemolysis is enabled by erythrocyte mass preliminary incubated in an equal volume of Krebs-Henseleit buffer pH 7.5 containing 20 % of DMSO, then frozen and stored under certain conditions; before the examinations, the samples are defrozen that is followed by haemolysis of the erythrocyte mass to 0.4 M in a trisHCl solution with centrifugal sedimentation of the membranes.;EFFECT: erythrocytic membranes produced by the method described above preserve their morphofunctional status.;1 tbl, 1 ex