METHOD OF PRODUCING RECOMBINANT HUMAN PROINSULIN C-PEPTIDE

摘要

FIELD: chemistry.;SUBSTANCE: invention relates to biotechnology, particularly genetic and protein engineering, as well as a method of producing recombinant human C-peptide. The disclosed method involves culturing an Escherichia coli producing strain, breaking down bacterial cells by disintegration, separating "inclusion bodies" containing a hybrid protein, dissolution thereof in a buffer containing urea and dithiothreitol, renaturating and purifying the renatured hybrid protein, disintegration thereof with trypsin and carboxypeptidase B, separation via chromatography on SP-sepharose, followed by purification and obtaining the end product.;EFFECT: method enables to obtain C-peptide with high output and purity of not less than 95% from wastes formed when producing recombinant human insulin.;4 tbl, 6 ex