A method for the qualitative and quantitative detection of specific nucleic acid sequences in real time

摘要

The method and a test kit is used for the qualitative and quantitative detection of nucleic acid sequences in real time. The subject matter of the invention is the use of a only with a fluorochrome (fluorophore) labelled dna - probe, wherein the labeled base with the fluorophore is always located in spatial proximity to a guanine base is located, and so, without the use of a quencher dye substance – the fluorophore quenched. By means of primers, under hybridization conditions to produce a mixture of duplexes, wherein the duplexes the target - zero 4,8,12,15,19-docosapentaenoic acid, positioned on the labeled oligonucleotide (probe), comprise. By addition of a polymerase with exonuclease activity is due, the this reason the labeled oligonucleotide (probe) between the fluorophore - marking and of the guanine - base and thus the quenching is cancelled, is a measurable fluorescence - signal is generated.