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BLOOD TYPE ANTIGEN OF DIFFERENT TYPE FOR DIAGNOSIS AND TREATMENT
BLOOD TYPE ANTIGEN OF DIFFERENT TYPE FOR DIAGNOSIS AND TREATMENT
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机译:诊断和治疗不同类型的血型抗原
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摘要
PROBLEM TO BE SOLVED: To provide a method for determining the presence of a blood type reactive antibody in the serum of a subject.SOLUTION: The method includes steps for: (a) preparing a collection of microbeads of different subtypes in which each subtype is coated with a different blood group antigen, (b) adding the serum from the subject to the collection of microbeads; (c) incubating the serum and the microbeads for sufficient time for anti-blood group antibodies in the serum to bind to the blood group antigens; (d) incubating the microbeads with at least one labeled ligand capable of specifically binding with the anti-blood group antibodies bound to the blood group antigens; and (e) detecting the presence of labeled ligand bound to the anti-blood group antibodies to determine the presence or absence of the reactive antibodies.
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