Animal models expressing luciferase under the control of the myelin basic protein promoter (MBP-LUCI), and use of models for the in vivo bioluminescence imaging

摘要

For the quantification and visualization in vivo at the transcriptional level remyelination events and demyelination in the CNS, myelin basic protein - luciferase bioimaging non-invasive model is provided. Were generated using (MBP) promoter myelin basic protein coupled to the firefly luciferase reporter transgenic animals expressing luciferase. MBP-luci bioimaging model provides a means for monitoring the efficacy of a test compound to modulate the remyelination and state of myelination. The advantage of bio-imaging, a subject that can serve as its own control in long-term studies. During the remyelination process and demyelination, the same subject may be tracked continuously over a period of 10 weeks at least. This model provides a good quality of the data distributed is reduced considerably and enables the normalization of the animal imaging individual response. Furthermore, it is not necessary to sacrifice at different time points of the cohort animals, it is possible to reduce the number required for the compound efficacy study.