Animal models expressing luciferase CONTROLLED PROMOTER myelin basic protein (MBP-LUCI), AND APPLICATION FOR VISUALIZATION MODELS IN VIVO bioluminescence

摘要

1. A method of monitoring demyelination or remyelination in vivo, wherein said method comprises: modifying said transgenic organism for the expression of the luciferase gene controlled by the promoter of myelin basic protein (MBP); monitoring the bioluminescence of the said body; ikorrelyatsiyu said light output with certain parts of the body indicated organizma.2. A method according to claim 1, wherein said model body is mlekopitayuschim.3. A method according to claim 2, wherein said mammal is mysh.4. A method according to claim 1, further comprising: repetition of the same body during said monitoring, characterized by monitoring said initial time, and comparing the output of said event monitor for observing changes in myelination in one organizme.5. A method according to claim 4, further comprising: normalizing the imaging signal over the intervals of demyelination and remyelination, said visualization during intervals effectively detects changes in transcript levels of MBP in said body for said intervalov.6. The transgenic organism containing a luciferase gene driven by promoter, myelin basic protein (MBP) .7. The transgenic organism according to claim 6, wherein said body is mysh.8. A transgenic organism expressing the luciferase gene, said luciferase gene under the control of a promoter of myelin basic protein (MBP) .9. A transgenic animal according to claim 8, wherein the model organism is mysh.10. The transgenic organism according to claim 6, wherein said promoter comprises MBP-M1-M4.11. The transgenic organism according to claim 6, wherein said promoter comprises MBP-