Colonization method of a support containing layer of polymeric nanofibers by bacterial or yeast population and the support containing a layer of polymeric nanofibers

摘要

ways of colonization nosice containing layer polymeric microfibers bacterial or yeast populations.in which the bacterial or fungal population prenese sterile media and incubated at kultivacnu00edho, while allowing for the induction of its exponential, respectively.stationary phase rustovu00e9 this bacterial or yeast population separated from kultivacnu00edho mediawashed with physiological saline and resuspended in bunecnou concentration corresponding to the optical densite 0,5.then is separated from the saline solution and inoculated into fresh sterile kultivacnu00edho media the same slozenu00ed as in the first step.and with this kultivacnu00edm medium and with nosicem containing layer polymeric microfibers are placed into the reactive vessel biological reactor, where the udrzuje moving relative to nosici,while allowing reaches of only natural links between bunkami bacterial or yeast population and polymer nanovlu00e1kny nosice.a layer of polymeric microfibers nosice long as after 48 hours will have colonized bacterial or yeast populations capable of further reproduction in mnozstvu00ed at least 0,05 mg / cm.sup.2.n.nosice.nosice containing layer of polymeric microfibers, which colonised at least one strain of bacteria or yeasts in a state capable of reproduction.