Colonization method of a support containing layer of polymeric nanofibers by bacterial or yeast population and the support containing a layer of polymeric nanofibers

摘要

A method of colonizing a carrier comprising a layer of polymeric nanofibers with a bacterial or yeast population, wherein said bacterial or yeast population is transferred to a sterile culture medium and cultured to yield its exponential and, respectively, exponential culture media. the stationary growth phase, this bacterial or yeast population is separated from the culture medium, washed with saline and resuspended to a cell concentration corresponding to an optical density of 0.5. It is then separated from the saline solution and inoculated into fresh sterile culture medium of the same composition as in the first step, and placed with the culture medium and the carrier containing the polymer nanofiber layer in a reaction vessel of the biological reactor where relative movement is maintained relative to the carrier. it produces a natural bond between the bacterial or yeast population cells and the polymer nanofibers of the carrier. The layer of polymeric nanofibres of the carrier is colonized after 48 hours by a bacterial or yeast population capable of further reproduction in at least 0.05 mg / cm.sup.2 of carrier. A carrier containing a layer of polymeric nanofibers that is colonized by a population of at least one strain of bacteria or yeast in a state capable of reproduction.