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Methods for packaging propagation-defective Vesicular Stomatitis Virus vectors
Methods for packaging propagation-defective Vesicular Stomatitis Virus vectors
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机译:包装传播缺陷的水泡性口腔炎病毒载体的方法
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摘要
A method of producing propagation-defective Vesicular Stomatitis Virus (VSV) in a cell culture is provided. The method involves introducing a plasmid vector encoding an optimized VSV G gene into a cell; expressing VSV G protein from the optimized VSV G gene; and introducing a propagation-defective VSV into the cell expressing the VSV G protein encoded by the optimized VSV G gene. The method further includes growing the cells in culture; and recovering the propagation-defective VSV from the culture.
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