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Methods for packaging propagation-defective Vesicular Stomatitis Virus vectors using a stable cell line that expresses G protein
Methods for packaging propagation-defective Vesicular Stomatitis Virus vectors using a stable cell line that expresses G protein
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机译:使用表达G蛋白的稳定细胞系包装传播缺陷的水泡性口腔炎病毒载体的方法
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摘要
A method of producing propagation-defective Vesicular Stomatitis Virus (VSV) is provided. The method involves providing a cell that includes an optimized VSV G gene, wherein expression of VSV G protein from the optimized VSV G gene is inducible; and inducing the cell to express VSV G protein from the optimized VSV G gene. The method also involves infecting the induced cell with an attenuated VSV; growing the infected cells in culture; and recovering attenuated VSV from the culture.
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