METHODS FOR PACKAGING PROPAGATION-DEFECTIVE VESICULAR STOMATITIS VIRUS VECTORS USING A STABLE CELL LINE THAT EXPRESSES G PROTEIN

摘要

A method of producing propagation-defective Vesicular Stomatitis Virus (VSV)is provided. The method involvesproviding a cell that includes an optimized VSV G gene, wherein expression ofVSV G protein from the optimized VSV G gene isinducible; and inducing the cell to express VSV G protein from the optimizedVSV G gene. The method also involves infecting theinduced cell with an attenuated VSV; growing the infected cells in culture;and recovering attenuated VSV from the culture.