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YEAST BIOETHANOL PRODUCTION STRAINS ALLOWING RAPID DETECTION OF WILD CONTAMINANT STRAINS

机译:可以快速检测野生污染物菌株的酵母生物乙醇生产菌株

摘要

The present invention relates to industrial yeast strains for bioethanol production that are marked specifically by insertion of the AGT1gene in a stable, well-conserved position in the genome. The resulting strains are organisms obtained by self-cloning. The insertion not only allows rapid and specific detection by PCR directly in ferment or samples of the production strain and of all wild, contaminant Saccharomyces cerevisiae strains, even when present in low amounts, but also improves the fermentation performance of the strain on starch hydrolysates and other maltose and/or maltotriose containing media.
机译:本发明涉及用于生产生物乙醇的工业酵母菌株,其通过在基因组中稳定,良好保守的位置插入AGT1基因而特别标记。所得菌株是通过自我克隆获得的生物。该插入不仅允许直接在生产菌株以及所有野生的,有污染的酿酒酵母菌株的发酵液或样品中进行PCR的快速和特异性检测,即使存在量很小,也可以提高该菌株在淀粉水解产物和淀粉发酵液中的发酵性能。其他含麦芽糖和/或麦芽三糖的培养基。

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