Method for detecting PON1 gene polymorphism (Q192R)

摘要

A probe for detecting a polymorphism in the PON1 gene, comprising at least one fluorescently labeled oligonucleotide selected from P1, P1', P2 and P2' below: (P1) an oligonucleotide comprising a nucleotide sequence of 6 to 53 consecutive nucleotides containing nucleotides 299 to 304 in SEQ ID NO: 1 or a homologous sequence thereof, wherein the nucleotide corresponding to the nucleotide at position 304 is a cytosine and is labeled with a fluorescent dye; (P1') an oligonucleotide comprising a nucleotide sequence of 6 to 53 consecutive nucleotides containing nucleotides 299 to 304 in SEQ ID NO: 1 or a nucleotide sequence which hybridizes with the complementary strand of the nucleotides 299 to 304 in SEQ ID NO: 1 under stringent conditions, wherein the nucleotide corresponding to the nucleotide at position 304 is a cytosine and is labeled with a fluorescent dye; (P2) an oligonucleotide comprising a nucleotide sequence of 12 to 54 consecutive nucleotides containing nucleotides 290 to 301 in SEQ ID NO: 1 or a homologous sequence thereof, wherein the nucleotide corresponding to the nucleotide at position 290 is a cytosine and is labeled with a fluorescent dye; and (P2') an oligonucleotide comprising a nucleotide sequence of 12 to 54 consecutive nucleotides containing nucleotides 290 to 301 in SEQ ID NO:1 or a nucleotide sequence which hybridizes with the complementary strand of the nucleotides 290 to 301 in SEQ ID NO: 1 under stringent conditions, wherein the nucleotide corresponding to the nucleotide at position 290 is a cytosine and is labeled with a fluorescent dye.