Probe for detecting polymorphism in CYP3A gene, method of detecting polymorphism, method of evaluating drug efficacy, and reagent kit for detecting polymorphism

摘要

Provided is a probe for detecting polymorphism that enables a simple detection of polymorphism in the CYP3A gene with high sensitivity. A probe which detects polymorphism in the CYP3A gene, the probe including: (P1) a fluorescently labeled oligonucleotide having a sequence including the 401st to the 411th bases of the sequence indicated in SEQ ID NO: 1 and having a length of 11 bases to 50 bases, the sequence having at least 80% identity with respect to a base sequence having the same bases as SEQ ID NO: 1 with the exception that the base corresponding to the 411th base in SEQ ID NO: 1 is cytosine, and the base corresponding to the 411th base of the sequence indicated in SEQ ID NO: 1 is labeled with a fluorescent dye; or (P1') a fluorescently labeled oligonucleotide having a sequence including the 401st to the 411th bases of the sequence indicated in SEQ ID NO: 1 and having a length of 11 bases to 50 bases, the sequence being capable of hybridization under stringent conditions to the complementary strand of a base sequence having the same bases as SEQ ID NO: 1 with the exception that the base corresponding to the 411th base in SEQ ID NO: 1 is cytosine, and the base corresponding to the 411th base of the sequence indicated in SEQ ID NO: 1 is labeled with a fluorescent dye.