PURPOSE: A stoichiometric fluorescent marker having thiol selectivity, a manufacturing method thereof, and an in-vivo thiol imaging method using the same are provided to show excellent light emittance, and to directly visualize thiol in the living body in real time. CONSTITUTION: A stoichiometric fluorescent marker has the thiol selectivity represented by chemical formula 1. A manufacturing method of the stoichiometric fluorescent marker having the thiol selectivity comprises a step of manufacturing a compound represented by the chemical formula 1 by performing deacetylation reaction on a compound represented by chemical formula 2. The compound represented by the chemical formula 2 is manufactured by reacting a compound represented by chemical formula 3 with the triphosgene and 2,2'-dithioethanol. The compound represented by the chemical formula 3 is manufactured by reacting a compound represented by chemical formula 4 with the galactose tetraacetate derivative having a -NH2 end group.
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