PRIMER COMPOSITION FOR LOOP-MEDIATED ISOTHERMAL AMPLIFICATION REACTION FOR DETECTING SWEET POTATO LEAF CURL VIRUS, AND USE THEREOF

摘要

PURPOSE: A primer composition for loop-mediated isothermal amplification reaction for detecting sweet potato leaf curl virus, and a use thereof are provided to quickly diagnose the virus using high concentration SYBR green I with naked eye. CONSTITUTION: A primer set for loop-mediated isothermal amplification reaction for detecting sweet potato leaf curl virus has sequence numbers 1-4. The primer set has sequence numbers 16-19. The virus is selected from the group consisting of Korean mutant strains(HM754635, HM754636, HM754641, and FJ560719), Brazil mutant strains(HQ393473), USA mutant strains(HQ333142), Japanese mutant strains(AB433788), Chinese mutant strains(JF768740), Chinese mutant strains(JF736657), Spain mutant strains(EU856364), and India mutant strains(NC_013640). A primer composition for loop-mediated isothermal amplification reaction for detecting sweet potato leaf curl virus contains the primer set. The composition further contains DNA polymerase, dNTPs, and a reaction buffer.