QUANTITATION METHOD OF EXOSOME IN A CELL CULTURE, AND A METHOD FOR ENHANCING YIELD OF EXOSOME BY THE SAME

摘要

PURPOSE: A quantitation method of exosome in a cell culture and a method for enhancing yield of exosome by the same are provided to enhance yield of exosome and to effectively use exosome.;CONSTITUTION: A method for enhancing detection efficiency of a fluorescent material in exosome comprises: a step of incubating protease and a cell culture medium containing recombinant exosome having a fluorescent material; and a step of filtering by a filter. A method for determining yield of exosome by the filter comprises a step of mixing the recombinant exosome and a sample containing exosome; a step of incubating the sample and protease; a step of filtering; and a step of measuring fluorescence in the filtered exosome and determining yield of exosome.;COPYRIGHT KIPO 2013;[Reference numerals] (AA) Background fluorescence; (BB) Washing numbers