ISOLATION METHOD OF ANTIOBESE CONSTITUENTS FROM YUKEUIGAMBITANG

摘要

PURPOSE: A method for isolating a fraction with an anti-obesity activity from Yukeuigambitang (kind of oriental medicine) is provided to obtain a food which prevents obesity by being added to an anti-obesity food. CONSTITUTION: A method for isolating a fraction with an anti-obesity activity from Yukeuigambitang comprises the steps of: extracting Yukeuigambitang containing Cinnamomum cassia Blume, adlay, Poria cocos, safflower, and Glycyrrhiza uralensis Fischer with ethanol; concentrating by reduced pressure; suspending in distilled water to obtain a suspension; extracting the suspension with dichloromethane and separating into a dichloromethane-soluble part and a dichloromethane-insoluble part; injecting the dichloromethane-soluble part onto MPLC (UV 254nm) and a redisep normal-phase flash column; sequentially developing with a solvent (CH_2Cl_2 : MeOH = 50:1-1:1) containing dichloromethane and methanol to sequentially fraction peaks detected at MPLC UV 254nm; and obtaining C1, C2, C3, C4, C5, C8, C9, and C10 fractions and obtaining C5, C6, and C7 fractions with an antiobesity activity among C1, C2, C3, C4, C5, C8, C9, and C10 fractions.