A METHOD FOR DERACEMIZATION OF HOMOALANINE USING D-AMINO ACID OXIDASE AND -TRANSAMINASE

摘要

PURPOSE: A deracemization method of D-amino acids by combination of vitreoscilla hemoglobin-D-amino acid oxidase (VHb-DAAO) and omega-transaminase (omega-TA) is provided to effectively produce optically pure L-homoalanine in an industrial size. CONSTITUTION: A deracemization method of D-homoalanine for producing L-homoalanine comprises the step of treating D-homoalanine with a fusion protein of vitreoscilla hemoglobin and VHb-DAAO, omega-TA, and an amino donor. The method comprises the steps of: adding isooctane to a phosphate buffer solution containing D-homoalanine, VHb-DAAO, omega-TA, flavin adenine dinucleotide (FAD) enzyme, benzyl amine, and catalase to prepare an organic and water-soluble biphasic reactant; and incubating the biphasic reactant. [Reference numerals] (AA) Catalase; (BB) Common product; (CC) Amino donor