New recombinant microorganisms comprising increased concentration of D-amino acid oxidase, L-amino acid dehydrogenase, co-substrate NADH enzyme and catalase than the parental microorganism, useful to prepare L-amino acids from D-amino acids

摘要

Recombinant microorganisms (I) (comprising a increased concentration of or activity of D-amino acid oxidase, L-amino acid dehydrogenase, an enzyme regenerating the co-substrate NADH and optionally catalase than the parental microorganism) is new. Independent claims are also included for: (1) a vector (A) comprising one or more of D-amino acid oxidase and amino acid dehydrogenase, for regenerating co-substrate NADH enzyme-coding nucleotide sequences; and (2) preparation of L-amino acids from D-amino acids using a enantioselective enzymatic synthesis, comprising adding (I) (comprising increased concentration of or activity of D-amino acid oxidase, L-amino acid dehydrogenase, an enzyme regenerating the co-substrate NADH and optionally catalase than the parental microorganism (e.g. wild type)) in solution containing the D-amino acids to convert the D-amino acids into L-amino acids; and isolating the formed L-amino acids.